M.W. 905.04

C47H68O17

[83314-01-6]

M.I. 13: 1442

Note: Binds to glass and plastic surfaces in aqueous solutions.

Storage:  Store at or below -20 ºC.  Solubility:  Soluble in DMSO or ethanol.  Disposal:  A

Bryostatin 1 binds to and activates protein kinase C, but its biological effects differ greatly from those induced by other protein kinase C activators.  Bryostatin 1 fails to mimic many effects caused by PMA and actually blocks some PMA-induced response in a variety of cells and tissues.  Kraft, A.S. et al. "Bryostatin, an activator of the calcium phospholipid-dependent protein kinase, blocks phorbol ester-induced differentiation of human promyelocytic leukemia cells HL-60." Proc. Natl. Acad. Sci. USA 83: 1334-1338 (1986).  Sako, T. et al. "Partial parallelism and partial blockade by bryostatin 1 of effects of phorbol ester tumor promoters on primary mouse epidermal cells." Cancer Res. 47: 5445-5450 (1987).  Dell'Aquila, M.L. et al. "Inhibition by bryostatin 1 of the phorbol ester-induced blockage of differentiation in hexamethylene bisacetamide-treated Friend erythroleukemia cells." Cancer Res. 47: 6006-6009 (1987).  Hess, A.D. et al. "Activation of human T lymphocytes by bryostatin " J. Immunol. 141: 3263-3269 (1988).

The true affinity of bryostatin 1 for PDBu binding site on PKC appears to be in the low picomolar range or lower.  de Vries, D.J., et al. "Demonstration of sub-nanomolar affinity of bryostatin 1 for the phorbol ester receptor in rat brain." Biochem. Pharmacol. 37: 4069-4073 (1988).

See technical Note #1 for additonal information.

Please note:  The correct molecular weight for bryostatin I is 905.04.  Perhaps the M.W. 887 error found in other catalogs arises because the original publication describing bryostatin I mentions a mass spectrum peak at 887, but apparently that peak resulted from the loss of one water molecule during the mass spectral process.