GENERAL INFORMATION

 

Price: 850 €

Cell System Components:

-Normal Human dendritic cells, cryopreserved > 2 500 000 cells/ampoule

 

Characterization of Cells

Cells are isolated from peripheral blood by leukopheresis and density gradient centrifugation. After four days in culture the cell stain >  90%  positive for CD11c and CD86 surface antigens and are < 5%  positive for  CD14 surface antigen using flow cytometric analysis. These  cell only produce IL12  P70 and no IL10. Non- routine characterization assays are available upon request for an additional fee.

 

Surface antigen                                          result

CD1a                                                  Positive

CD3                                                    Negative

CD4                                                    Positive (dim, low level expression)

CD8                                                    Negative

CD11c                                               Positive

CD14                                                 Negative

CD19                                                 Negative

CD80                                                 Low to absent

CD83                                                 Low to absent, regulated by IL1β & INFα

CD86                                                 Positive

C54                                                    Positive

FcRγI                                                 Negative

FcRγII                                                Positive

FcRγIII                                               Negative

FcRα                                                 Low to absent

 

INDICATION

 

Viability upo plating from Cryo:  > 85%

Recommended plating density:  20 000-50 000 cells/cm²

Viable time in culture: 7 days

 

This product is for research use only. Not approved for humans or veterinary use, or for in vitro diagnostic or clinical procedures.