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Domoic Acid ImmunoSepDomoic Acid ImmunoSepTM Introduction Methods that use immunoaffinity chromatography (IAC) for sample preparation or detection are becoming increasingly popular as tools in the analytical applications. Besides, there is the possibility to combine this technique with others instrumental techniques. Several applications of immunoaffinity chromatography sample preparation are described in literature, applications such as: drug, foods, environmental analysis, micotoxins, natural toxins, etc. The immunoaffinity columns are prepared by binding specific antibodies to specially activated solid support and packing the support suspended in aqueous buffer solution into a cartridge. The analytes in the extracts or fluid binds to the antibody, impurities are removed with washing buffer solution, and then the analites are eluted with a adequate solvent for elution. Further the analysis of eluates can be done by different techniques (HPLC, GC, Electrophoresis, etc.) ImmunoSepTM pack for Analysis of ASP toxins Amnesic shellfish poisoning (ASP) is caused by consumption of different species of shellfish that have accumulated domoic acid, a neurotoxin produced by diatom. The neurotoxin properties of domoic acid result in neuronal degeneration and necrosis in specific regions of the hippocampus. Monitoring programs are in place in numerous countries worldwide and closures of shellfish harvesting areas occur when domoic acid levels exceed regulatory limits. Control mechanisms have been introduced in order to carry out monitoring phytoplankton and shellfish products, and different analytical tools have been proposed for the control of this toxin. The high affinity and the high selectivity of the antigen antibody interactions for domoic acid and its isomers allow the specific extraction and the concentration of this toxic analyte previous to instrumental analysis by HPLC-UV or HPLC-MS. Advantages use Domoic Acid ImmunoSepTM: Simplifies the sample preparation procedures for Domoic acid and its isomers Selective removes interference from complex matrices The columns can be regenerated without lack of efficiency (optimization studies) Allows an environmentally friendly technique and eliminates the use of chlorinated solvents Greatly reduces the amounts of organic solvents required (only small quantities of methanol are required) High and reproducible recovery results of the analytes from complex matrices Columns Specification: PE syringe capacity: 1 mL Solid support: 100 mg / column (Activated Sepharose) Preservation solution: PBS buffer pH 7,4 with 0,5% sodium azide Immunosorbent: Anti-domoic acid (DA) monoclonal antibody Antibody Cross-reactivity: Domoic acid isomers Columns per pack: 2 Samples: Waters, algae extracts, shellfish, fish
Domoic Acid ImmunoSepTM Application: ImmunoSepTM immunoaffinity columns have been developed and adapted for use with complex matrices to selectivity extract several potential additives and contaminants, in food, water and biological matrices.
Product reference Domoic acid ImmunoSep™ is a new generation of solid phase extraction (SPE) device where the retention mechanism is very specific, involving antibody-antigen interactions. Domoic Acid ImmunoSep™ was designed for multiresidue analysis of ASP toxins and may be adapted to many different matrices. This SPE-IAC device is a sepharose-based sorbent, allowing a higher resistance to medium constituents. A choice of more hydrophilic solid support is provided to the user to meet complex matrices of different interfering nature. Catalogue # S0250 Quality Control Samples of IAC columns are going to be submitted to guarantee tests of quality control using HPLC-UV and HPLC-MS techniques. Availability Available in stock in May 2005, for more information please contact AbKem Iberia SL. (info@abkemiberia.com or sales@abkemiberia.com)
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