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Chemical Product and Company Identification |
Name |
G418 Sulfate Cat# 81-108321-42-2 |
Synonyms |
Antibiotic G-418, Geneticin disulfate salt, 108321-42-2 |
Hazards |
Health: 1 Flammability: 0 Reactivity: 0 |
Company Identification |
GENTAUR LABORATORIES Avenue de l'Armée 68 1040 Brussels, BELGIUM Tel: +32 2 732 56 88 Fax: + 32 2 732 44 14 e-mail: info@gentaur.com |
In case of Emergency |
Centre Antipoisons (Brussels) Tel: +32 (0) 70 245 245 |
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Identity
Information |
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CAS Registry Number |
108321-42-2 |
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UN (DOT) |
1541 |
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Physical/Chemical
Characteristics |
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Molecular Formular |
C20H40N4O10.
2H2SO4 |
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Structure |
 |
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Appearance |
Odourless, white to
off-white powder |
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Molecular weight |
692.7 |
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Potency
|
> 650µg/mg |
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BIOLOGICAL POTENCY TEST ED50
Sensitivity assay |
≤400 mg / ml |
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BIOLOGICAL POTENCY TEST ED50
Resistant assay |
≥2,500 mg / ml |
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SPECIFIC ROTATION: (C=1% in H2O at
25°C) |
+104° - +121° |
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ELEMENTAL ANALYSIS: (Based on 4H2O) |
C: 32.3 ± 2.4 %, H:
6.7 ± 0.35 %, N: 7.5 ± 0.55 % |
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TLC |
Max. Three spots |
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ABSORBANCE570 nm (100 mg / ml) |
< 0.10 |
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ABSORBANCE280 nm (1 mg / ml) |
< 0.015 |
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Solubility |
Water: 50mg/ml |
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Moisture content |
< 10.0% |
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AMMONIA |
< 1.0 % |
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Preparation
Instructions |
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Working
concentration |
Since this
antibiotic is toxic for many cells, one has to determine the
optimal concentration for each cell type. |
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Recommended => < 5 µg /ml for bacteria and
algae |
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=> 300 – 500
µg/ml for animal cells |
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~ 400 µg/ml needed
for selection and ~ 200 µg/ml for maintenance in mammalian cells |
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Storage/ Stability |
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Powder |
Storage
temperature 2-8°C |
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Warning |
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For Research Use
Only. Not for drug, household or other uses. See Material Safety
Data Sheet for additional information. |
