MAGNETIC SEPARATORS
Spherotech has several different
designs of magnetic separators. These are used for separating both
Paramagnetic and Ferromagnetic particles from a suspension. These
designs accommodate different size tubes and other receptacles specific
to various applications.
(A) The SPHEROTM FlexiMag Separator (Cat. No.: FMS-1000) accommodates
tubes ranging from 1.5 mL microfuge tube to 200 mL tissue culture
bottle. It uses interchangeable tube holders to secure different size
tubes and bottles. The separator comes with a set of three tube holders,
Small, Medium and Large. Additional holders can be purchased separately,
if needed.
a) The Small holder holds up to four
1.5 mL microfuge or four 10 or 12x75 mm test tubes (8 total).
b) The Medium holder can hold two 15
mL centrifuge or two 16x100 mm tubes (4 total)
c) The Large holder can hold two 50 mL
centrifuge tubes (4 total).
d) Two 200 mL tissue culture bottles
directly without any tube holders.
(B) The SPHEROTM FlexiMag Separator
Jr. (Cat. No.: FMJ-1000) holds up to eight 1.5 mL microfuge tubes, 5 mL
cryovials, 10x75 mm or 12x75mm tubes.
(C) The SPHEROTM MicroMag Separator
(Cat No. MMS-2100 and MMS-2000) are designed to fit any 96-well plate
with round bottom, flat bottom or V bottom.
(D) The SPHEROTM HandiMag Separator is
a 1"x2"x0.375" Neodymium-Iron-Boron high strength magnet with nickel
coating . The HandiMag can be used to separate the magnetic particles in
various containers such as microfuge tubes, test tubes or centrifuge
tubes by holding the magnet against the wall of containers by hand or
with a rubber band.
(E) The SPHEROTM MiniTube Mag
Separator (Cat. No.: MTMS-16) holds up to sixteen 1.5 mL tubes.
(F) The SPHEROTM UltraMag Separator
(Cat. No.: UMS-3000) and SPHEROTM UltraMag DW Separator
(Cat. No.: UMDS-1000) are designed to
facilitate the washing of magnetic particles in the Magnetic Particles
Enzyme Immunoassay (MPEIA) using
96-well plates. The magnetic pegs of UltraMag Separator fit between the
wells underneath the 96-well plate. The microplate is read in an
appropriate microtiter plate reader by loading the microplate with the
attached UltraMag Separator into the reader. The magnets ensure that the
magnetic particles stay out of light beam passing through the bottom of
the well and corresponding holes in the UltraMag Separator.
A combination of magnetic particles as
solid phase and conventional enzyme immuno assay (EIA) microplate
technology offers significant advantages over the conventional EIA. Some
of the advantages are listed below:
Magnetic particles offer larger surface area and significantly faster
reaction kinetics; thus, reducing the total time to complete an assay.
Microparticles are washed more
efficiently leaving less residual reactants, thus, lowering background
signal and potentially improving sensitivity.
Coating of magnetic particle is
easier, gives a more uniform solid phase and helps to minimize
lot-to-lot variation.
See the MAGNETIC SEPARATOR section of
the TECHNICAL INFORMATION Page of the Spherotech website to see photos
of several magnetic separators.
