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Chemical Product and Company Identification |
Name |
DNA Polymerase, TAQ Cat # 11-BIO-21-40 |
Uses |
DNA
amplification via PCR |
Product features |
Highly
processive 5'–3' DNA Polymerase |
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Extremely
thermostable |
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Recombinant
enzyme for best reproducible results
|
Hazards |
Health: 1 Flammability: 0 Reactivity: 0 |
Company Identification |
GENTAUR LABORATORIES Avenue de l'Armée 68 1040 Brussels, BELGIUM Tel: +32 2 732 56 88 Fax: + 32 2 732 44 14 e-mail: info@gentaur.com |
In case of Emergency |
Centre Antipoisons (Brussels) Tel: +32 (0) 70 245 245 |
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Identity Information |
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CAS Registry Number |
9012-90-2 |
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EC (EINECS/ELINCS) |
232-741-2 |
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Physical/Chemical Characteristics
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Concentration |
5 Unit/ul, 500Units/vial |
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Included |
10X reaction buffer and 25mM MgCl2 buffer |
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Appearance |
White to brown coloured liquid |
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Odour |
Typical enzyme odour |
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Sources |
Thermus aquaticus |
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Error rate |
1/105 |
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Unit definition |
One unit incorporates 10 nmole of dNTP into
acid-insoluble material in 30 min. at 74°C. |
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QUALITY
TESTING |
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Our Thermostable DNA Polymerase is highly purified, free
of contaminating endonucleases, exonucleases and nicking activity. For
endonuclease assay, 1 ug of Lambda/Hind III DNA is incubated with 20
units of the enzyme in assay buffer at 75°C for 16 hrs and no visible
contaminating activity is observed; For exonucleases assay, 1 ug of
pBR322 plasmid DNA is incubated with 10 units of enzyme for 16 hrs at
75°C in assay buffer and no detectable exonuclease is observed. The
purity of the enzyme is also evaluated by adding 10 units of DNA
polymerase in 100 ul of a reaction mixture for making first strand cDNA
at beginning and no impaired effect on the first strand is observed. |
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10X
REACTION BUFFER(Without MgCl2) |
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500 mM KCl, 100 mM Tris HCl (pH 9.0) at 25°C, 1% Triton
X-100 Buffer is optimized for use with 200 uM dNTPs |
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25mM
MgCl2 BUFFER |
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In general, 1.5-2.5 mM final concentration of MgCl2
is recommended. |
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*The final MgCl2 concentration may be
variable according to individual experiment requirements. |
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STORAGE
BUFFER |
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20 mM Tris HCl ( pH 8.0 ), 0.l mM EDTA, lm M DTT, 0.1%
Triton X-100, 50% glycerol |
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EXTENSION CHARACTERISTIC |
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Our Thermostable DNA Polymerase has the independent
terminal transferal activity which results in the addition of a single
nucleotide ( adenosine ) at 3' end of the extension product. TA cloning
vector is recommended if the extension product is needed to be cloned. |
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Storage |
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Storage
Temperature |
Store at -20°C and keep tightly closed. |
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Warning |
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For Research Use Only. Not for drug, household or other
uses. See Material Safety Data Sheet for additional information. |
