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GENTAUR Tel: +32 16 58 90 45 Fax: + 32 16 50 90 45 Av. de l'Armée 68 • B-1040 BRUSSELS • BELGIUM
GENTAUR FRANCE Tel: 01 43 25 01 50 9, Rue Lagrange • 75005 PARIS • FRANCE |
Human soluble MICA ELISA Kit Antigen: Soluble human MICA (MHC-class I-related chain A) Catalog Number: MICA-EK-1000Applications: Detection of soluble MICA in serum and cell culture supernatantsCapture antibody (1 vial): Clone: AMO1 (mouse IgG1, kappa) Concentration: 1 mg/ml Formulation: 0.5 mg in 0.5 ml phosphate-buffered saline, pH 7.4, no sodium azide Storage: Store at 4°C. For long-term storage freezing at -20°C is recommended. Detection antibody (1 vial): Clone: BAMO3 (mouse IgG2a, kappa) Concentration: 0.2 mg/ml Formulation: 0.1 mg in 0.5 ml phosphate-buffered saline, pH 7.4, no sodium azide Storage: Store at 4°C. For long-term storage freezing at -20°C is recommended. Standard (5 vials): recombinant soluble MICA*04 (purified from E.coli)Concentration: 100 ng/ml Formulation: 20 ng in 0.2 ml phosphate-buffered saline, 5% bovine serum albumin, pH 7.4 Storage: Store at -20°C. Use aliquots after thawing within 1-2 weeks. Usage: See attached ELISA instruction sheet.Description: MICA (MHC class I-related chain A) is a polymorphic, human MHC-encoded cell surface glycoprotein and ligand of the activating C-type lectin-like immunoreceptor NKG2D [1-5]. NKG2D engagement of MICA activates NK cells and costimulates CD8 T cells [3,6]. MICA is expressed on gastrointestinal epithelium and inducible by cell stress, viral and bacterial infection [2,6-8]. MICA is also expressed by malignant epithelial and haematopoietic cells, and MICA expression has been shown to enhance tumor rejection in vivo [9-12]. Tumor cells shed soluble MICA which is detectable in sera of patients with epithelial and haematopoietic malignancies and may counteract tumor immunosurveillance [10,13-15].
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