Chromogenic in situ
hybridization (CISH)
CISH is a
practical, cost-effective, and valid
alternative to fluorescent in
situ hybridization (FISH) and
can be easily integrated into
routine testing in laboratories.
Compared to FISH chromogenic in
situ hybridization offers three
important advantages:
1. the histological details of the
paraffin section are generally
better appreciated with a bright
field
2. the morphological details are
readily apparent using low-power
objectives
3. the probe signals are not subject
to rapid fading
(Chang et al. HER-2/neu
oncogene amplification by
chromogenic in situ hybridization in
130 breast cancers using tissue
microarray and clinical follow-up
studies. J Korean Med Sci 2004;
19: 390-396)
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| CISH analysis of
paraffin embedded
tissue using the
ZytoFast
®
HPV-Typing
system (T-1028). HPV
6/11 infected cells
are indicated by a
dark blue/violet
precipitate. |
|
ZytoFast in situ
hybridization products
The
ZytoFast
system uses tagged
oligonucleotide probes which are
indirectly detected using an
enzyme-conjugated antibody targeting
the tags. The enzymatic reaction of
chromogenic substrates leads to the
formation of colour precipitates
that can be visualized by light
microscopy. The result is achieved
within just 4 hours!
Click on the product groups below
for more information
